Yes, he did. During the Giants' Week 7 OT win over Houston, Skattebo was flagged for unnecessary roughness (lowering his helmet at the second level) on a successful 7-yard run against Texans safety M.J. Stewart. Harbaugh visibly confronted the officiating crew on the sideline, and he then delivered a full-throated defense of Skattebo's running style on Monday.
Harbaugh argued the call conflicts with how RBs have been taught to play for decades: "You have to run low to protect the football. You have to run low to protect your body. You can't run in there and give tacklers your chest. When you run low, your head's down there too. You can't move your head off your shoulders. It's a physical impossibility." He added Skattebo "tries to keep his head in a good, strong upright position... he runs low to the ground. That's a good thing." (from the Cam Skattebo and John Harbaugh pages)
Skattebo himself was also fired up post-game, criticizing the NFL's mixed messaging: "the NFL loves to post me when I do stuff like that, but then also wants to take stuff from me."